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Candida albicans - consensus
A Consensus Candida albicans MLST scheme
was established to provide a reference scheme for typing
of C. albicans and
to allow unambiguous comparison of data between different
laboratories. This new scheme uses a set of 7 internal fragments
from different house-keeping genes (AAT1a, ACC1, ADP1, MPIb,
SYA1, VPS13, ZWF1b), 4 of which (ACC1, ADP1, SYA1, VPS1)
were part of the original 6-genes
scheme. This 7 gene set should become the international standard
for C. albicans MLST.
Primers for the amplification and sequencing of the seven
gene fragments can be obtained here.
The consensus C. albicans MLST scheme has
been developed by Marie-Elisabeth Bougnoux in the laboratory
of Christophe
d’Enfert (Fungal Biology and Pathogenicity Unit at
Institut Pasteur, Paris, France. ), and
Arianna Tavanti in the laboratory of Frank
Odds (Department
of Molecular & Cell Biology at Institute of Medical Sciences,
University of Aberdeen, Aberdeen, United Kingdom. ).
The initial database contains the profiles of 279 clinical
isolates and will be updated with new profiles at intervals.
Those performing MLST on this species are encouraged to
submit their data to the curator (M.E.
Bougnoux)
so that the strain details can be added to the database.
The seven loci and the primers used for
the consensus C. albicans MLST
The consensus C. albicans MLST scheme uses internal fragments
of the following seven genes :
AAT1: Aspartate aminotransferase
ACC1: Acetyl-coenzyme A carboxylase
ADP1: ATP-dependent permease
MPI: Mannose phosphate isomerase
SYA1: Alanyl-RNA synthetase
VPS13: Vacuolar protein sorting protein
ZWF1b : Glucose-6-phosphate dehydrogenase
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Locus
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Amplicon
size (bp)
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Sequenced fragment size(bp)
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Primer
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AAT1a
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478
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349
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Fwd
5’-ACTCAAGCTAGATTTTTGGC- 3’
Rev
5’-CAGCAACATGATTAGCCC- 3’
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ACC1
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519
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407
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Fwd 5’-GCAAGAGAAATTTTAATTCAATG- 3’
Rev 5’-TTCATCAACATCATCCAAGTG- 3’
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ADP1
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537
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443
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Fwd 5’-GAGCCAAGTATGAATGATTTG- 3’
Rev 5’-TTGATCAACAAACCCGATAAT- 3’
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MPI b
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486
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375
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Fwd 5’-ACCAGAAATGGCCATTGC- 3’
Rev 5’-GCAGCCATGCATTCAATTAT- 3’
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SYA1
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543
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391
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Fwd 5’-AGAAGAATTGTTGCTGTTACTG- 3’
Rev 5’-GTTACCTTTACCACCAGCTTT- 3’
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VPS13
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741
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403
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Fwd 5’-TCGTTGAGAGATATTCGACTT- 3’
Rev 5’-ACGGATGGATCTCCAGTCC- 3’
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ZWF1b
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702
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491
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Fwd 5’-GTTTCATTTGATCCTGAAGC- 3’
Rev 5’-GCCATTGATAAGTACCTGGAT- 3’
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